Isolation, molecular identification, and in vitro control of Campomanesia rufa (O. Berg) Nied. endogenous bacteria

ABSTRACT Campomanesia rufa (O. Berg) Nied. is a Myrtaceae species native to the Brazilian Cerrado. As C. considered endangered, in vitro propagation an alternative for its conservation. However, lack of effective disinfection protocols endophytic microorganisms naturally present plant tissues hinders success micropropagation. In this context, objective study was isolate, molecular identify, and control endogenous occurring bacteria propagated vitro. Purified PCR products bacterial isolates were sequenced by Sanger method aligned with homologous sequences using Basic Local Alignment Search Tool (BLAST) available National Centre Biotechnology Information (NCBI) database. MEGA7 software used align all draw phylogenetic trees. Survival, shoot height, efficiency antibiotics (streptomycin, ampicillin, chloramphenicol) at different concentrations culture medium evaluated. Different Bacillus sp. strains observed tissues. When testing antibiotics, 32 mg L-1 ampicillin caused significant reduction contamination no effect on explant survival. On other hand, 256 streptomycin greatest but lethal over 90% explants. This first report occurrence their context